anti rat igg2a af488  (SouthernBiotech)

Journal: iScience

Article Title: Miniaturized Fab’ imaging probe derived from a clinical antibody: Characterization and imaging in CRISPRi-attenuated mammary tumor models

doi: 10.1016/j.isci.2024.110102

Figure Lengend Snippet:

Article Snippet: Sections were washed 5× in 5% calf serum in TBS and incubated in the presence of a mixture of Cetuximab-AF647 (1.5 μg/mL), anti-rat IgG2a-AF488 ( SouthernBiotech , diluted 1:50) and Cy3-labeled anti-digoxigenin Fab ( Millipore-Sigma , concentration – 0.5 μg/mL) in 5% calf serum in TBS for 1 h. Sections were washed 5× in 5% calf serum in TBS, fixed in 4% formaldehyde in DPBS, pH 7.5 for 10 min and mounted using Vectashield with DAPI ( Vector Labs ).

Techniques: Recombinant, Protease Inhibitor, Hybridization, In Vivo, Fluorescence, Software, CRISPR, Plasmid Preparation, Purification, cDNA Synthesis